Fungi associated with PBSA and Mung Bean (Vigna radiata L.) Raw sequence reads

Bio-based and biodegradable plastics such as mulching films are widely used in agricultural field sites. However, there are limited studies of their impact on plant development and health even though an important soil-borne plant pathogen F.solani has been reported to associate with various types of bio-based and biodegradable plastics, especially polybutylene succinate-co-adipate (PBSA). To evaluate the influence of PBSA addition in soils on plant development and health, F. solani and mung bean (V. radiata) were used as models in a modified petri-dish test using soil suspensions. Mung bean seeds were incubated in suspensions with different dilutions of soils pre-incubated 1 year with PBSA under different treatments (combinations of N fertilizer (ammonium sulfate) and PBSA load) in the modified petri dish test. Plant development and disease incidence were recorded with both microscopic and molecular techniques (specific PCR and Illumina amplicon sequencing). Treatment with PBSA and N fertilizer in non-sterile soil suspensions strongly increased the disease caused by F. solani onV. radiata at both low and high soil dilution. At high soil dilution, the F. solani disease incident was 67.5% while at the low dilution the disease incidence reached 92%. In contrast, in treatments PBSA but without N fertilizer, non F. solani diseasewas observed. Apart from F. solani infection, other soil fungi can also infect the mung bean seedlings, especially at low soil dilution levels. Nevertheless, based on this short-term study, we found no evidence that PBSA alone can significantlyincrease the overall disease incidence. In this project, we provided raw sequence data of fungi associated with soil pre-incubated 1 year with PBSA with N fertilizer (ammonium sulfate) (SPN treatment) and V. radiata seedlings incubated in SPN treatment.