Growth characteristics of mutant, reintegrant, and wild-type strains on different carbon sources at 30°C.

Cells were pregrown to mid-log phase in YPD, washed, resuspended in water to an OD600 of 0.5 (about 8.5×106 cells/ml), and serially diluted fivefold. Drops of 7 µl of each dilution were deposited onto solid minimum media (YNB), containing 2% of either glucose, acetate, glycerol, or fatty acid as the sole carbon source. Growth was observed after 3 days (glucose) or 5 days (acetate, glycerol, fatty acids) of incubation at 30°C. Growth on the first dilution (1∶5) was noted ‘+’, on the second dilution (1∶52) was noted ‘++’, on the third dilution (1∶53) was noted ‘+++’, on the fourth dilution (1∶54) was noted ‘++++’, on the fifth dilution (1∶55) was noted ‘+++++’, and no growth was noted ‘−’.Growth characteristics of mutant, reintegrant, and wild-type strains on different carbon sources at 30°C.