Dyadic encounter unfamiliar female pairs over 2 days – 16p11.2 Del mouse strain – WT-WT - 5202_5191

We evaluated the social interactions and communication between unfamiliar individuals in pairs. For these recordings of social behaviour and ultrasonic communication, we focused on pairs of individuals since we currently cannot identify the emitter of USVs when animals were interacting closely. Therefore, we recorded undisturbed dyadic interactions between two unfamiliar individuals (from two different housing cages) of the same age (14-20 weeks of age) and genotype for 47h (two days and nights). For that purpose, we coupled the Live Mouse Tracker system (plugin 931) with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration > 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy < 50%; Avisoft Bioacoustics, Glienecke, Germany). LMT and Avisoft systems were synchronised based on the protocol described in (de Chaumont et al., 2021). Each upload includes the sqlite database from LMT (processed, with USV synchronised) and a zip file with all USVs sequences automatically recorded by Avisoft.本研究对成对陌生个体间的社会互动与交流行为进行了评估。鉴于当前动物近距离互动时，无法识别超声发声（Ultrasonic Vocalizations, USVs）的发声源，因此本次针对社会行为与超声波通讯的记录仅聚焦于成对个体。 为此，本研究对来自不同饲养笼、周龄一致（14~20周）且基因型相同的两只陌生个体，开展了时长47小时（包含两个昼夜）的无干扰成对互动记录。为完成该记录，本研究将活体小鼠追踪系统（Live Mouse Tracker，插件931）与1台CM16/CMPA型麦克风（德国格利尼克Avisoft生物声学公司）相连接，该麦克风接入Avisoft Ultrasound Gate 416超声采集设备，其参数设置为：采样率300 kHz、16位采样格式；触发条件：该通道电平；预触发时长1秒；保持时长1秒；有效时长＞0.005秒；触发事件：25~125 kHz频段内能量占比达2%且熵值＜50%；该设备由德国格利尼克Avisoft生物声学公司提供。 活体小鼠追踪系统（LMT）与Avisoft采集系统依据de Chaumont等人2021年发表的研究中所述的同步协议完成同步。每份上传内容均包含来自LMT的SQLite数据库（已完成处理且超声发声同步），以及包含Avisoft自动录制的全部超声发声序列的压缩包。