Dyadic encounter unfamiliar female pairs over 2 days – 16p11.2 Del mouse strain – Del/+-Del/+ - 5187_5230

We evaluated the social interactions and communication between unfamiliar individuals in pairs. For these recordings of social behaviour and ultrasonic communication, we focused on pairs of individuals since we currently cannot identify the emitter of USVs when animals were interacting closely. Therefore, we recorded undisturbed dyadic interactions between two unfamiliar individuals (from two different housing cages) of the same age (14-20 weeks of age) and genotype for 47h (two days and nights). For that purpose, we coupled the Live Mouse Tracker system (plugin 931) with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration > 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy < 50%; Avisoft Bioacoustics, Glienecke, Germany). LMT and Avisoft systems were synchronised based on the protocol described in (de Chaumont et al., 2021). Each upload includes the sqlite database from LMT (processed, with USV synchronised) and a zip file with all USVs sequences automatically recorded by Avisoft.

本研究成对评估了陌生个体间的社会互动与交流行为。针对社会行为与超声通讯的录制工作，我们仅选取成对个体作为研究对象，原因在于当前无法在动物近距离互动时识别超声发声（Ultrasonic Vocalizations, USVs）的发声个体。因此，我们对来自不同饲养笼、年龄一致（14~20周龄）且基因型相同的两只陌生个体间的无干扰成对互动，进行了为期47小时（两个昼夜）的录制。为此，我们将活体小鼠追踪系统（Live Mouse Tracker，LMT，插件931）与1支CM16/CMPA型麦克风（德国格利尼克Avisoft Bioacoustics公司生产）相连，并将麦克风接入Avisoft Ultrasound Gate 416超声采集设备（采样率300千赫，16位量化格式；触发条件：该通道电平；预触发时长1秒；保持时长1秒；有效时长>0.005秒；触发事件：25~125千赫频段内能量占比2%且熵值<50%，德国格利尼克Avisoft Bioacoustics公司生产）。LMT与Avisoft系统基于de Chaumont等人2021年发表的实验方案完成同步校准。每份上传数据均包含经处理且已完成USV同步的LMT sqlite数据库文件，以及由Avisoft自动录制的全部USV序列压缩包。