aThe total volume of PCR reaction was 25 µl and the PCR conditions were as follows: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C, 15 s for denaturation and 60°C, 1 min for annealing

NIAID Data Ecosystem20

Primer sequences used for mRNA quantification by real-time PCR.

基因表达定量

实时PCR

11β-HSD1, 11β-hydroxysteroid dehydrogenase type 1; 5αR1, 5α-reductase type 1; 5βR, 5β-reductase; GR, glucocorticoid receptor; C/EBPα, CCAAT/enhancer-binding protein α; C/EBPβ, CCAAT/enhancer-binding p

Figshare2015-12-02 更新10

qPCR primer sequences.

qPCR引物序列

基因表达定量

qPCR primer sequences.

NIAID Data Ecosystem30

The primer sequences and positive controls of pathogens.

病原体分子检测

引物设计

The primer sequences and positive controls of pathogens.

Figshare2021-08-16 更新20