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Surveyor assay for three guide RNAs targeting near R332 and R335
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创建时间：
2018-01-14
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sgRNAs used in this study and CRISPR targeting efficiency measured by TIDE analysis.
Genomic DNA was harvested from CRISPR-targeted and non-targeted cells and the targeted locus was amplified by PCR (~200bp upstream and downstream of the predicted cut site), and sequenced via capillar
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