Expression of 97 surface markers and 462 mRNAs in 31586 cells from 15 leukemic human bone marrow

Seurat v3 object<b><br></b><b>ASSAYS</b>:AB: Antibody expression dataRNA: mRNA expression dataintegrated: Antibody and mRNA expression matrices were concatenated and data was integrated across patients using scanorama<br><b>DIMENSIONALITY REDUCTION</b>MOFA: Multi-OMICS factor analysis to integrate AB and RNA data. MOFA was run on scanorama-integrated data and served as input for clustering and further dimensionality reduction.MOFAUMAP: UMAP performed on MOFA dimensions. <b>Projected:</b> Cell projected into the MOFAUMAP space of a healthy dataset. Display used in the manuscript.<br><br><b>METADATA</b>ct: Cell typeBatch: Source individual (AML1-3, AML Q1-Q6, APL Q1-Q6)<br>Patient Identifier age gender GenotypeAML Q1 AML116 71 f FLT3-ITD,NPM1-mutAML Q2 AML327 62 f FLT3-wt,NPM1-mutAML Q3 AML127 56 f FLT3-wt,NPM1-mutAML Q4 AML314 51 m FLT3-ITD,NPM1-mutAML Q5 AML334 78 m FLT3-wt,NPM1-mutAML Q6 AML183 61 m FLT3-wt,NPM1-mutAML1 AML1 44 f FLT3-ITD,NPM1-mutAML2 Q59 61 f FLT3-wt,NPM1-mutAML3 AML3 55 m FLT3-ITD,NPM1-mutAPL Q1 APL224 70 f APLAPL Q2 APL223 54 f APLAPL Q3 APL142 66 m APLAPL Q4 APL147 45 m APLAPL Q5 APL124 32 m APLAPL Q6 APL218 46 f APL