The ninhydrin assay is an interesting alternative for protein quantification to the widely used Kjeldahl and elemental analysis methods. The two latter methods only measure the nitrogen content that m
a) Detection of JUP-81 by immunoblotting with mAb 2C9. b) Semi-quantitative analysis of Western blots. JUP plasma levels were correlated to JUP in a reference plasma that was run on each blot.
The goal of the project was to quantify the dynamics of BRD4 proline hydroxylation in response to oxygen depletion and differential enzyme regulations.
A fast and accurate assay to determine the absolute concentration of proteins is described based on direct measurement of droplet entrapped oligomer formation in electrospray. Here we demonstrate the