3d structure of the human dentate gyrus by holo-tomography: Alzheimer disease vs Control

The goal of the experiment is a full 3d quantitative analysis of the dentate gyrus (DG) region in human hippocampal tissue, and pathological changes of the 3d structure due to Alzheimer’s disease (AD). In particular, we firstly want to test the hypothesis that AD is accompanied by changes in neuron structure, density and packing in the DG, and secondly whether amyloid plaques are linked by filamentous connections. For this purpose, we will use the unique capabilities of ID16a as a world leading instrument for full field 3d high resolution phase contrast, in combination with advanced iterative phase retrieval and segmentation algorithms.