Identifying signatures of EV secretion in metastatic breast cancer through functional single-cell profiling

Extracellular vesicles (EVs) regulate the tumor microenvironment by facilitating transport of biomolecular cargo including RNA, protein, and metabolites. We developed an integrated method to identify individual cells with differences in EV secretion and linked single-cell RNA-sequencing on cloned single cells from MDAMB231 cell line. Additionally, We profiled parental MDAMB231, MCF7 and HCC70 cell lines. Single-cell RNA sequencing of two monoclonal cell lines (MDAMB231-S with high and MDAMB231-NS with low EV secretion), parental MDAMB231, MCF7, and HCC70 cell lines.