Real-time quantitative PCR analysis of differentiated U937 cells stimulated with LPS+IFNgamma. Real-time quantitative PCR analysis of differentiated U937 cells stimulated with LPS+IFNgamma

U937 monocyte cell line was differentiated using PMA. Differentiated U937 monocytes were exposed to MEM+10% FBS medium (untreated cells) and the same medium containing LPS+IFNgamma. The changes in the gene expression of cytokines and chemokines were evaluated using RT2 Profiler qPCR array Overall design: Cells from three wells of 6WP were pooled and RNA was extracted from cells for both conditions. One array plate was used per condition.