Rubus wahlbergii and related species in Sweden

We studied Rubus wahlbergii and related species in R. sect. Corylifolii, primarily in Sweden, using DNA microsatellites. The results show that the material can be divided into three species: R. wahlbergii s.s. which occurs along the Swedish coast from Bohuslän to Uppland, R. nordicus which occurs on the western coast all the way south to north-western Skåne, and a previously unrecognized taxon which we here describe as R. wahlbergioides. The latter has scattered localities in Halland, Skåne, Blekinge, Öland and Småland, but is common around Kivik in Skåne and Kalmar in Småland. It is characterised by leaves that are without felt underneath, terminal leaflets with a somewhat more triangular shape and coarser serration than those of R. wahlbergii and also has more glands on the stem and the inflorescence. In the northern part of the distribution, R. nordicus is easily distinguished morphologically from R. wahlbergii, e.g. by a strong tendency to form 7-foliolate leaves, but in Skåne and H..., Samples (young leaves from the tip of the annual shoot) of R. wahlbergii and several related Rubusspecies were collected, mainly from Sweden, but also from Denmark, Norway and Germany. The localities are specified in Table S1 in the Supplementary Material. Vouchers are deposited in LD. The samples were stored in a freezer at –80˚C until DNA was extracted.
DNA was extracted with the 2× CTAB method (Doyle and Doyle, 1987). The concentration of the DNA was determined by a fluorometer and the samples were diluted with ddH2O to ~14 ng/µL. Four pairs of microsatellite primers, described in Table S2, were used to analyse variable microsatellite loci. The three loci Ru105b, Ru117b and Ru275a were described in R. idaeus (Graham et al., 2004), whereas the locus mRaCIRRIV2A8 was described in R. alceifolius Poir. (Ansellem et al., 2001). They will here be referred to as 105b, 117b, 275a and 2A8, respectively. They have been successfully used also for other Rubus species (Stafne et al., 2005; Zhou, ..., , # Rubus wahlbergii and related species in Sweden

[https://doi.org/10.5061/dryad.3bk3j9ktf](https://doi.org/10.5061/dryad.3bk3j9ktf)

Mingyue Zhou, Melanie S. Montes, Tore Mattsson, Tomas Burén, Mikael Hedrén & Ulf Ryde (2024)
Nordic J. Bot., in press; DOI: 10.1111/njb.04373

**Explanation of the columns:**
A Suggested species according to the investigation (text)
B DNA bank number - Unique extraction number (integer)
C Collection code - Unique identification code (text)
D Genus - Always \"Rubus\" (text)
E Collected as - species name at collection (text)
F Habitat (text)
G Province, mainly in Sweden (text)
H Parish (text)
I Locality (text)
J x coordinate (N/S) in Swedish RT90 (integer)
K y coordinate (E/W) in Swedish RT90 (integer)
L Date I - date of first observation or collection (year-month-day)
M Voucher no - unique for each date (integer) - only if a herbarium voucher is collected, otherwise empty
N Date II - date of collection, if different from Data I (year-month-day)
O Collec...

本研究以瑞典为核心研究区域，对榛叶悬钩子组（Sect. Corylifolii）中的瓦氏悬钩子（Rubus wahlbergii）及其近缘类群开展了基于DNA微卫星（DNA microsatellites）标记的研究。

研究结果显示，供试材料可划分为3个物种：一是狭义瓦氏悬钩子（R. wahlbergii s.s.），分布于瑞典沿海区域，范围从布胡斯兰（Bohuslän）延伸至乌普兰（Uppland）；二是北欧悬钩子（R. nordicus），分布于瑞典西海岸，向南直至斯科讷（Skåne）西北部；三是此前未被识别的新类群，本文将其命名为拟瓦氏悬钩子（R. wahlbergioides）。

拟瓦氏悬钩子在哈兰（Halland）、斯科讷、布莱金厄（Blekinge）、厄兰（Öland）与斯莫兰（Småland）区域有零星分布，但在斯科讷的基维克（Kivik）周边以及斯莫兰的卡尔马（Kalmar）周边较为常见。该类群的形态特征为：叶背无绒毛，顶生小叶相较于瓦氏悬钩子呈更显著的三角形，叶缘锯齿更为粗糙；茎秆与花序上的腺点也更多。

在分布区北部，北欧悬钩子可凭借显著的7出复叶特性，在形态上与瓦氏悬钩子轻松区分，但在斯科讷和H……地区，原文此处存在截断。

本研究采集了瓦氏悬钩子及其他数种悬钩子属近缘物种的样本，样本取自一年生枝条顶端的幼叶，采集地主要位于瑞典，同时也涵盖丹麦、挪威与德国。详细采样地点信息见补充材料中的表S1。凭证标本存放于LD标本馆。所有样本均在-80℃冰箱中冷冻保存，直至开展DNA提取。

DNA提取采用2×CTAB法（Doyle与Doyle，1987）。使用荧光计测定DNA浓度，随后用双蒸水（ddH₂O）将样本稀释至约14 ng/μL。本研究使用4对微卫星引物对可变微卫星位点进行分析，引物详情见表S2。其中Ru105b、Ru117b与Ru275a这3个位点最初在红树莓（R. idaeus）中被报道（Graham等，2004），而位点mRaCIRRIV2A8则是在大花悬钩子（R. alceifolius Poir.）中被描述（Ansellem等，2001）。本文将上述位点分别简称为105b、117b、275a与2A8。此前已有研究将这些引物成功应用于其他悬钩子属物种（Stafne等，2005；Zhou等，……）。

# 瑞典境内的瓦氏悬钩子及其近缘类群
[https://doi.org/10.5061/dryad.3bk3j9ktf]
作者：周明月（Mingyue Zhou）、Melanie S. Montes、Tore Mattsson、Tomas Burén、Mikael Hedrén与Ulf Ryde（2024）
《北欧植物学杂志》（Nordic J. Bot.），即将出版；DOI: 10.1111/njb.04373

**列项说明：**
A：本研究建议的物种名称（文本型）
B：DNA样本编号——唯一提取编号（整数型）
C：采集编码——唯一识别编码（文本型）
D：属名——固定为"悬钩子属（Rubus）"（文本型）
E：采集时定名——采集时记录的物种名称（文本型）
F：生境（文本型）
G：省份（主要为瑞典境内省份，文本型）
H：教区（文本型）
I：采样点（文本型）
J：瑞典RT90坐标系的x坐标（南北向，整数型）
K：瑞典RT90坐标系的y坐标（东西向，整数型）
L：首次观测或采集日期（格式：年-月-日，文本型）
M：凭证标本号——单日唯一编号（整数型），仅当采集了腊叶凭证标本时填写，否则留空
N：第二次采集日期——若与首次观测/采集日期不同则填写（格式：年-月-日，文本型）
O：采集信息（原文此处截断为Collec...）