Intraesophageally challenge of OVA to epicutaneously sensitized mice with loss of epithelial Ikkβ results in experimental EoE that is faithful to human EoE esophageal mucosal pathology. [I]

We report a novel mouse model that can faithfully mimic human EoE EoE: Three to four month old Cre+; IKKBL/L mice and littermate controls (IkkβL/L) were treated topically on the ears for 14 days with 2nmol of MC903 (Tocris Bioscience) and 100 μg of OVA , once daily. On day 15 and 17, mice were challenged intraesophageally (IE) with 50 mg OVA. Sensitization: Cre+;IkkβL/L mice and littermate (IkkβL/L) genetic controls were treated on the ears with a combination of MC903 and OVA and gavaged with water. Vehicle: Cre+;IkkβL/L mice and littermate genetic controls were treated on the ears with a combination of ethanol and PBS and gavaged with water.