Details of the multiple sequence alignment (MSA) methods used and maximum likelihood phylogenies estimated from them.

We define the core length as the number of character positions from the first to the last of the characteristic cysteine residues (C1–C6) of the OBPs. The log-likelihoods (LnL), parsimony informative characters, tree size, and average approximate likelihood-ratio tests (aLRT) are derived from the ML analyses. Robinson-Foulds tree distances (RF distance) are calculated by comparing the ant and bee MAP trees to the ML trees derived under the other MSA methods. Best scores of the MSAs compared to the BAli-Phy MAP are in highlighted in bold italics.