Continuous culture enrichments from intertidal sediment - Metagenomes, Metatranscriptomes and 16S tag sequencing

A continuous culture was inoculated with intertidal sediment from the German Wadden Sea, and introduced with artificial seawater and a mimicked tidal condition. Natural selection on the microbial community and redox processes were investigated in the culture. Three samples for DNA extraction and four samples for RNA extraction were taken from the culture during the last three weeks of the experiment. Whole DNA shotgun metagenomes were sequenced and the reads were assembled into contigs. Enriched total mRNA was used for transcriptome sequencing. Another 14 samples at different time points were taken for 16S tag sequencing.