Quantification of thiol pool in S. meliloti 1021 and Bradyrhizobium sp after 30 minute stress with SNP in media.

S. meliloti cells were grown to mid log phase at 30°C in TY media whereas Bradyrhizobium sp cells were grown similarly in YEM media. Sodium nitroprusside (SNP) was added to mid log phase cell in the final concentration of 1.0 and 3.0 mM and incubated at 30°C for 30 minute. Cells were harvested, lysed and cell free crude extracts were subjected to quantification of total thiol, oxidized glutathione, reduced glutathione and ratio for reduced to oxidized glutathione. Results are expressed as mean ± SD, for n = 3 experiments. P≤0.01, using one-way ANOVA.