A high-throughput skim-sequencing approach for genotyping, dosage estimation and identifying translocations

An optimized, high-throughput and cost-effective genotyping method applicable to various crop breeding populations is very important in this genomic era. We have developed an optimized Nextera skim-sequencing (skim-seq) approach to genotype different populations that can be used for genetics studies and genomics-assisted breeding. We performed skim-seq on a variety of populations developed through doubled haploid (DH) technology, inter-specific recombinants developed through introgression, amphidiploid developed through wide crosses, and on known monosomic samples.

1. A doubled haploid (DH) population consisting of 48 lines from the cross of spring wheat (Triticum aestivum) cultivars CDC Stanley and CDC Landmark developed by the Crop Development Centre at the University of Saskatchewan. We genotyped these DH with skim-seq and identified the genomic segments contributed by each of the two parental lines.

2. A population of 335 back cross generation 1 (BC1) skim-seq samples for whea...