CIL:34521

The video is a representative movie of baceria incubated in DMEM for 3 h on top of an array of pilars of stiffness 500 pN/micron. The video is real time and shows the behavior of one or two bacteria for 23 s after 3 h of incubation. The video relates to CIL: 34522 and 34523, which show bacteria incubated in different media. The pilars are 3 micron apart center to center. The video is DIC microscopy with the focus on the top of the pilars. See the already deflected pillars around the bacterium and both transient and sustained pulls. This video corresponds to Video 2 from PLoS Biol. 2008 Apr 15;6(4):e87.本视频为在刚度为500皮牛每微米的微柱阵列表面，经德式改良伊格尔培养基（DMEM）孵育3小时的细菌的代表性影像。该视频为实时录制，记录了孵育3小时后1至2株细菌在23秒内的行为状态。本视频关联CIL:34522与CIL:34523两组影像，后者展示了在不同培养基中孵育的细菌。微柱的中心间距为3微米。本视频采用微分干涉差（DIC）显微镜成像，对焦于微柱顶端。可观察到细菌周围已发生偏转的微柱，以及瞬态与持续的牵拉现象。本视频对应《公共科学图书馆·生物学》（PLOS Biology）2008年4月15日刊第6卷第4期e87文章中的视频2。