Tubuloside B alleviates inflammation of murine macrophages

Purpose: Studies in mice have indicated that Tubuloside B has therapeutic potentials in various models of inflammatory diseases. We set out to analyze how Tubuloside B regulates transcription in LPS+IFN-?-stimulated macrophages. Methods: RNA-seq was performed with two repetitions in RAW264.7 followed by treatment with Tubuloside B and LPS as well as IFN-?. Conclusions: Tubuloside B inhibits the production of pro-inflammatory cytokines in activated macrophages. Overall design: RNA-seq was performed with two repetitions in RAW264.7 followed by treatment with Tubuloside B and 10 ng/ml LPS as well as 20 ng/ml IFN-? for 24 h. The gene expression profile was studied by RNA sequencing.