Photodynamic therapy with infracyanine green induces keratocyte depopulation in the normal equine cornea

The purpose of this study was to investigate the morphologic effects and safety of in vivo photodynamic therapy (PDT) on the healthy equine cornea. Six university-owned horses underwent unilateral corneal PDT with intrastromal infracyanine green and photoactivation with an 810nm diode laser (500mW for 2.5 min = 75 Joules). Complete ophthalmic examinations, clinical scoring, digital and infrared photography, ocular thermography, in vivo confocal microscopy (IVCM), and ultrasound biomicroscopy (UBM) were performed pre-treatment and post-treatment on study days 1, 5, 15, 33, and 103.
, , # Data from: Photodynamic therapy with infracyanine green induces keratocyte depopulation in the normal equine cornea

Dataset DOI: [10.5061/dryad.xgxd254w6](https://doi.org/10.5061/dryad.xgxd254w6)

## Description of the data and file structure

Six horses underwent unilateral corneal photodynamic therapy with intrastromal infracyanine green and photoactivation with an 810nm diode laser (500mW for 2.5 min = 75 Joules). Complete ophthalmic examinations, clinical scoring, digital and infrared photography, ocular thermography, in vivo confocal microscopy (IVCM), and ultrasound biomicroscopy (UBM) were performed pre-treatment and post-treatment on study days 1, 5, 15, 33, and 103.

Clinical scoring parameters were based on a scoring system modified from the semiquantitative preclinical ocular toxicology scoring (SPOTS) system (Eaton et al, J Ocul Pharacol Ther. 2017). The treated eye of each horse underwent graded assessment of pupillary light reflexes, conjunctival hyperemia, chemosis, oc..., ,

本研究旨在探讨活体光动力疗法（photodynamic therapy，PDT）对健康马角膜的形态学影响及安全性。6匹大学饲养的受试马接受单侧角膜光动力疗法，采用基质内注射吲哚菁绿（infracyanine green），并使用810nm二极管激光进行光激活（功率500mW，照射时长2.5分钟，总能量75焦耳）。分别在治疗前以及治疗后第1、5、15、33、103个研究日，对受试马开展全面眼科检查、临床评分、数码及红外摄影、眼温成像、活体共聚焦显微镜（in vivo confocal microscopy，IVCM）检测以及超声生物显微镜（ultrasound biomicroscopy，UBM）检测。

数据来源：《使用吲哚菁绿光动力疗法诱导正常马角膜基质细胞减少》（Photodynamic therapy with infracyanine green induces keratocyte depopulation in the normal equine cornea）
数据集DOI：[10.5061/dryad.xgxd254w6](https://doi.org/10.5061/dryad.xgxd254w6)

## 数据与文件结构说明
6匹马接受单侧角膜光动力疗法，采用基质内注射吲哚菁绿，并用810nm二极管激光进行光激活（功率500mW，照射时长2.5分钟，总能量75焦耳）。分别在治疗前及治疗后第1、5、15、33、103个研究日，开展全面眼科检查、临床评分、数码及红外摄影、眼温成像、活体共聚焦显微镜（IVCM）与超声生物显微镜（UBM）检测。
临床评分参数基于改良自半定量临床前眼毒理学评分（semiquantitative preclinical ocular toxicology scoring，SPOTS）系统的评分体系（Eaton等，《Journal of Ocular Pharmacology and Therapeutics》，2017年）。对每匹马的患眼进行分级评估，包括瞳孔对光反射、结膜充血、球结膜水肿、……