Characteristics of LHON cases and unaffected relatives recruited in the study.
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*MtDNA/nuclear DNA from fibroblast of each individual was measured according to Pejznochova M, 2008 [62] by amplification of ND5 gene in mitochondrial genome from position 13466–13650 (GenBank sequence NC_012920 gi:251831106), and PARL gene from nuclear genome from 16912–17165 (GenBank sequence: NC_000003.12 gi:224589815). Real-time PCR amplification was performed on Bio-Rad CFX 96 thermo cycler and the threshold cycle (Ct) was obtained. MtDNA/nuclear DNA ratio was calculated from the Ct (mtDNA)/Ct (nDNA) ratio. The increasing ratio shows decrease in amount of mtDNA per cell [62].Characteristics of LHON cases and unaffected relatives recruited in the study.
创建时间：
2015-12-02
