Dynamic Reorganization of Chromatin Accessibility Signatures during Dedifferentiation of Secretory Precursors into Lgr5+ Intestinal Stem Cells

To define differences in chormatin accessibility and histome modifications in intestinal stem and epithelial cells, we performed ATAC-seq and ChIP-seq. We used RNA-sequencing to profile gene expression changes during intestinal stem cell differentiation into different cell types. We find that gene expression is correlated with the chromatin accessibility and reveals differences between intestinal stem cells types. ATAC-seq, H3K4me1, H3K4me2 and H3K27ac ChIP-Seq analysis of isolated mouse intestinal stem cells and progenitor cells. RNA seq analysis of mouse intestinal stem, progenitor and differntiated cells.