Do it yourself: 3D-printed miniature CDC trap for adult mosquito (Diptera: Culicidae) surveillance

The central component of mosquito and vector surveillance programs globally is the adult mosquito trap, which is intended to collect host-seeking mosquitoes. The miniature CDC trap is a widely distributed trap style in part due to its relative affordability and compact nature. Despite already being a simple trap, in-house production methods, such as 3D printing, could improve the accessibility of the CDC trap by eliminating some of the supply chain variables. We present here several trials with the Salt Lake City (SLC) trap, a three-dimensional (3D) printed trap design. Functional assessments were made on secondary components and found no statistically significant differences when comparing CO2 line height (above vs. below fan), battery types (sealed lead acid vs. USB battery pack), and trap body collection shape (funnel body vs. simple/straight body). The SLC trap was compared directly to a commercial equivalent, the ABC trap, with comparative assessment on species diversity and evenne..., A prototype trap design, referred to as the SLC trap, was developed from a variety of exploratory model tests that are found in supplemental figures (Suppl. Fig. 1). Essentially, the prototype is a 1-piece tubular trap body with a rain guard (Fig. 1A). A 6-volt variable speed motor (RF-500TB-14415-R-J/32, Nichibo Taiwan Corp., Taipei, Taiwan) was equipped with a 4-blade, 7.5-cm propeller (Item #3C078RSHC1, Thorgren Tool & Molding, Inc., Valparaiso, IN) and secured inside the trap body with #2 conduit hanger (#2 ACC Conduit Hanger, Halex/Scott Fetzer Co, Cleveland, OH). All trap models can be operated using 18-guage, Class 2 copper wire (16-strand dual conductor/182C-200SR, MaxBrite LED Lighting Tech LLC, San Jose, CA). All 3D-printed components were fabricated from PLA plastic filament extruded through a 0.6 mm nozzle with set temperature at 210 °C (410 °F) and 58 °C (136 °F) bed temperature. The comparison model was the Clarke (or Pacific Biologics, AUS) branded ABC trap, with the ..., , # 3D\_printed\_cdc\_trap\_validation

A prototype 3D printed trap design, referred to as the SLC trap, was developed from a variety of exploratory model tests. Field sites were selected from historical surveillance locations of the Salt Lake City Mosquito Abatement District (SLCMAD) in environments containing a mixture of wetlands and sagebrush. Pairwise tests were conducted with different supplies and components to optimize the base model SLC trap.

## Description of the data and file structure

Trap_Development_data_DRYAD.xlsx

Airstone Height:

Positioning of the airstone for CO2 dispersal either above or below the downdraft fan. Data as aggregate mosquito collections for a 24 hr trap night per replicate using 1kg dry ice in a transport cooler and dispersed with a line to the trap body. Standard error of the mean included. Paired t-test results included.

Variables: Rep = replicate number; Airstone Height = height of airstone; Location = site ID for trap rotations; # Mosquitoes = p...

全球蚊媒监测项目的核心组件为成蚊诱捕器，其设计目标为采集觅宿主成蚊。微型CDC诱捕器（CDC trap）是目前应用范围最广的诱捕器类型之一，这在一定程度上得益于其相对经济的成本与紧凑的结构。尽管该诱捕器本身已较为简易，但通过3D打印等自制生产方式，可消除部分供应链变量，进一步提升CDC诱捕器的可及性。本研究针对盐湖城诱捕器（SLC trap）——一款三维（3D）打印的诱捕器设计方案——开展了多组试验。我们对其二级组件进行了功能评估，结果显示：二氧化碳管线高度（风扇上方与风扇下方）、电池类型（密封铅酸电池与USB充电宝）以及诱捕器本体收集腔形状（漏斗形与直筒形）之间均无统计学显著性差异。随后将SLC诱捕器与商业同款产品ABC诱捕器（ABC trap）进行直接对比，评估其物种多样性与均匀度……
一款名为SLC诱捕器的3D打印原型诱捕器设计，源自补充图（Suppl. Fig. 1）中展示的多组探索性模型试验。该原型整体为一体式管状诱捕器本体，搭配防雨罩（图1A）。所使用的6伏变速电机（型号RF-500TB-14415-R-J/32，Nichibo台湾公司（Nichibo Taiwan Corp.，中国台湾台北））搭载了一片7.5厘米的四叶螺旋桨（型号Item #3C078RSHC1，Thorgren Tool & Molding公司（Thorgren Tool & Molding, Inc.，印第安纳州瓦尔帕莱索）），并通过#2型导管挂架（型号#2 ACC Conduit Hanger，Halex/Scott Fetzer公司（Halex/Scott Fetzer Co.，俄亥俄州克利夫兰））固定于诱捕器本体内部。所有诱捕器型号均可使用18号线规的2类铜线（型号16股双导线/182C-200SR，MaxBrite LED Lighting Tech LLC公司（MaxBrite LED Lighting Tech LLC，加利福尼亚州圣何塞））进行供电。所有3D打印组件均采用PLA塑料丝材，通过0.6毫米喷嘴挤出，打印时喷嘴温度设置为210℃（410℉），热床温度设置为58℃（136℉）。对照模型为克拉克（或澳大利亚Pacific Biologics品牌）ABC诱捕器，……
# 3D_printed_cdc_trap_validation

一款名为SLC诱捕器的3D打印原型设计，源自多组探索性模型试验。试验场地选自盐湖城灭蚊区（Salt Lake City Mosquito Abatement District, SLCMAD）的历史监测点位，周边环境兼具湿地与蒿属灌丛。我们针对不同耗材与组件开展了配对试验，以优化基础款SLC诱捕器的设计。

## 数据与文件结构说明
Trap_Development_data_DRYAD.xlsx
气石高度：
用于二氧化碳扩散的气石安装位置分为下吸式风扇上方与下方两种。试验采用1kg干冰置于运输冷藏箱内，通过管线连接至诱捕器本体进行扩散，每组重复试验的24小时诱捕夜的成蚊捕获总量作为统计数据，包含均值标准误，同时附带配对t检验结果。
变量说明：Rep=重复试验编号；Airstone Height=气石高度；Location=诱捕轮换点位编号；# Mosquitoes=蚊子数量……