Additional file 1 of METTL1 promotes tumorigenesis through tRNA-derived fragment biogenesis in prostate cancer [Dataset]

Additional file 1: Supplementary Figure S1. Expression of RMPs in prostate tumours. A) mRNA expression of RMPs is significantly altered in human normal prostate tissue (N), primary (PT), and metastatic tumour (M). Y-axis shows log2-normalised expression data from Grasso et al. [7],Taylor et al. [6], and Varambally et al. [42] datasets (left panels). Kaplan–Meier curves showing disease-free survival (DFS) of patient groups selected according to decile expression of the indicated RMP genes (middle panel). The right panel shows differential expression in disease-free (DF) and recurrent patient samples from TCGA dataset [3]. B, C) Kaplan–Meier curves representing the disease-free survival (DFS) of patient groups selected according to the decile expression, data from the TCGA dataset [6] (TCGA: n = 491). D, E) PCa cell lines show increased METTL1 protein and mRNA levels. Western blotting showing METTL1, WDR4, AR, PTEN and phosphor-S6K expression (D), and qPCR (E) of normalised mRNA expression (lower panel) in prostate epithelial cell lines (PWR-1E and RWPE-1), benign prostatic hyperplasia cell lines (BPH1), and prostate cancer cell lines (VCap, C4-2, LNCaP, 22Rv1, DU145, and PC3). Mean ± SD (D, E). Statistical tests: ANOVA test (A), log-rank Cox test (A, B); Student’s t-test with Welch’s correction, grouping all data from benign vs. all data from tumour cells ****p < 0.0001 (E).