Next Generation Sequencing analysis at single-cell level of normal and degenerated nucleus pulposus cells transcriptomes

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Overall design: nucleus pulposus cells mRNA profiles of five degenerated and four normal human