Richard Allen (University of Hawaii) (2011) CIL:21697, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

Quick-freeze deep-etch image of a fracture through the radial canal of the contractile vacuole. This technique presents a picture of a confusing array of non-etchable material surrounding a collecting canal. Lumens of smooth spongiome tubules can be observed. One gets the impression that these tubules are not entirely smooth but that they do have subunits of some sort on their cytosolic surfaces. A few segments of decorated tubules with helically wound subunits on their cytosolic surfaces are visible at the periphery of this radial arm. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:13119) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

快速冷冻深蚀裂隙图像，展示了收缩性液泡径向管道中的断裂。该技术呈现出一幅周围环绕着难以蚀刻材料的复杂阵列，其中可见平滑海绵状管腔的管腔。观者会感觉到这些管腔并非完全平滑，其细胞质表面似乎存在某种亚单元。在径向臂的边缘，可见一些装饰性管腔的片段，其细胞质表面具有螺旋状缠绕的亚单元。该透射电子显微镜（TEM）图像由R. Allen于1992年5月26日使用Zeiss 10A在80kV下拍摄。负片放大倍数为19,800倍。标尺长度为0.2微米。负片的打印件已扫描并使用Photoshop进行处理。此图像最适宜进行定性分析。高分辨率图像（CIL:13119）可用于定量分析。更多详细信息请访问（http://www5.pbrc.hawaii.edu/allen/）。