SRE4 Effects of hydrocarbons on Antarctic Marine Sediment Ecosystems - SRE4_Microbial

Sediment Recruitment Experiment 4 (SRE4) was a large, long term (5 year) field experiment run at Casey Station (from 2001 to 2006) testing the effects of 4 different hydrocarbons on marine sediment ecosystems. Four different types of hydrocarbons were individually mixed with defaunated marine sediments and deployed in trays on the seabed at O'Brien Bay-1. Trays were collected after deployment periods of 5 weeks, 56 weeks, 62 weeks, 2 years and 5 years. In addition there was a bioturbation treatment using the burrowing urchin Abatus (at 56 weeks only). Samples were collected from 4 replicate trays of each treatment at each sampling time. Analyses were done of sediment hydrocarbon chemistry, microbial communities, meiofaunal communities, macrofaunal communities and diatom communities. The hydrocarbon treatments were: a synthetic Mobil lubricating oil; the same Mobil lubricating oil after 125? hours use in a vehicle engine; a Fuchs synthetic lubricating oil marketed as highly biodegradable; and Special Antarctic Blend diesel fuel (SAB). A control uncontaminated sediment treatment was used for comparison.

沉积物招募实验4（Sediment Recruitment Experiment 4，缩写SRE4）是一项大型长期（为期5年）野外实验，于2001年至2006年在凯西站（Casey Station）开展，旨在探究4种不同烃类物质对海洋沉积物生态系统的影响。研究人员将4类不同烃类物质分别与经脱除底栖生物处理的海洋沉积物混合后，装入托盘布放于奥布莱恩湾1号（O'Brien Bay-1）的海床之上。分别于布放5周、56周、62周、2年及5年后回收托盘。此外还设置了一项生物扰动处理组，仅在布放56周时使用掘穴海胆Abatus（Abatus）开展扰动实验。每次采样时，均从每个处理组的4个重复托盘中采集样本。针对沉积物烃类化学特征、微生物群落、小型底栖生物群落、大型底栖生物群落及硅藻群落开展了分析检测。本次实验的烃类处理组包括：美孚（Mobil）合成润滑油；经车辆发动机使用125小时后的同款美孚合成润滑油；福斯（Fuchs）合成润滑油（宣传为高生物降解型）；以及南极专用混合柴油（Special Antarctic Blend diesel fuel，缩写SAB）。同时设置未受污染的沉积物对照组用于对照比较。