Real-time quantitative PCR analysis of differentiated U937 cells stimulated with LPS+IFNgamma

U937 monocyte cell line was differentiated using PMA. Differentiated U937 monocytes were exposed to MEM+10% FBS medium (untreated cells) and the same medium containing LPS+IFNgamma. The changes in the gene expression of cytokines and chemokines were evaluated using RT2 Profiler qPCR array Cells from three wells of 6WP were pooled and RNA was extracted from cells for both conditions. One array plate was used per condition.