The data processing code and demonstration video of this article

This dataset provides raw impedance signals, processed waveform data, calibration factor calculations, and size distribution results for a full‑waveform calibration method used to eliminate position‑dependent errors in double‑differential impedance flow cytometry. It includes data from 10 μm/15 μm/1 mm microspheres and live/dead HEK‑293T cells, together with COMSOL simulation results and height‑sensing validation data. The dataset is accompanied by data processing code and a demonstration video, supporting the reproducibility of impedance signal filtering, event segmentation, full‑wave calibration, and particle size/height analysis.