Biotin Electron Diffraction (MicroED) Datasets - Glacios TEM with a CETA-D

Electron diffraction datasets collected from nanometer sized crystals of biotin (TCI, MDL Number: MFCD00005541) applied to an electron microscopy grid covered in a pure carbon film. Data were collected using a Thermo Fisher Scientific Glacios Transmission Electron Microscope operated at 200 kV and -193 C using the software package Leginon (Cheng, et. al. 2021). The beam diameter is ~600 nm and the total dose is ~3.4e-/A^2. The crystals were continuously rotated and diffraction data was collected with a CETA-D camera in rolling shutter mode. Data have been converted to SMV format with the addition of an offset value to remove negative pixel values. This offset value can be found in the image headers, along with a suggested pedestal value. An image of the crystal before centering on the small green dot is also included. Data from four crystals are provided along with data reduction results. If processing in DIALS, you will first want to install the proper format class: dxtbx.install_format -u https://raw.githubusercontent.com/dials/dxtbx_ED_formats/master/FormatSMVCetaD_TUI.py A data processing tutorial is available for these datasets using DIALS: https://dials.github.io/documentation/tutorials/3DED/Biotin.html本数据集采集自涂布于覆有纯碳膜的电镜载网上的生物素（Biotin，TCI品牌，MDL编号：MFCD00005541）纳米级晶体。数据采集使用赛默飞世尔科技（Thermo Fisher Scientific）Glacios型透射电子显微镜，加速电压设定为200 kV，样品台温度维持在-193℃，并通过Leginon软件包完成数据采集（Cheng等，2021）。电子束直径约为600 nm，总电子剂量约为3.4 e⁻/Ų。实验过程中晶体持续旋转，衍射数据通过搭载卷帘快门模式的CETA-D相机采集得到。所有数据已转换为SMV格式，为去除负像素值添加了偏移量；该偏移量可在图像头文件中查询，同时附带推荐的基座值。数据集还包含晶体在对准小型绿色标记点前的成像图。本数据集包含4组独立晶体的衍射数据，以及配套的数据约简结果。若使用DIALS进行数据处理，请先安装对应的格式类：执行命令 dxtbx.install_format -u https://raw.githubusercontent.com/dials/dxtbx_ED_formats/master/FormatSMVCetaD_TUI.py。针对本数据集的DIALS数据处理教程可通过以下链接获取：https://dials.github.io/documentation/tutorials/3DED/Biotin.html