Age-Associated B Cells Express a Diverse Repertoire of VH and Vκ Genes with Somatic Hypermutation - Data

Antibody sequences associated with the manuscript "Age-Associated B Cells Express a Diverse Repertoire of VH and Vκ Genes with Somatic Hypermutation" Lisa M Russell Knode, Martin S Naradikian, Arpita Myles, Jean L Scholz, Yi Hao, Danya Liu, Mandy L Ford, John W Tobias, Michael P Cancro, and Patricia J Gearhart. The Journal of Immunology. 2017 Mar 1;198(5):1921-1927. doi: 10.4049/jimmunol.1601106." Sequences were generated by RT-PCR using 3' Igh/Igk constant region oligos with 5' degenerative variable region oligos, sequences were generated by Sanger sequencing.

本数据集关联于论文《年龄相关性B细胞表达携带体细胞超突变的VH（Variable Heavy）与Vκ（Variable Kappa）基因多样化免疫组库》(Age-Associated B Cells Express a Diverse Repertoire of VH and Vκ Genes with Somatic Hypermutation)，作者为Lisa M Russell Knode、Martin S Naradikian、Arpita Myles、Jean L Scholz、Yi Hao、Danya Liu、Mandy L Ford、John W Tobias、Michael P Cancro及Patricia J Gearhart，发表于《免疫学杂志》(The Journal of Immunology)，2017年3月1日，第198卷第5期，页码1921-1927，DOI:10.4049/jimmunol.1601106。该数据集的抗体序列通过逆转录聚合酶链式反应（RT-PCR，Reverse Transcription Polymerase Chain Reaction）扩增获得：使用3'端Igh/Igk恒定区域寡核苷酸（oligos）引物与5'端简并可变区域寡核苷酸（oligos）引物进行扩增，随后通过Sanger测序（Sanger sequencing）得到最终序列。