'HoneySweet' Plum leaf small RNAs

The question was if there are specific small RNAs that are responsible for the high resistance to Plum Pox Virus, of the transgenic plum, 'HoneySweet', which has the coat protein gene for PPV. 'HoneySweet' plum trees in the field were exposed to Plum Pox Virus Rec strain through a graft of infected 'Emma Leppermann'. After five years the graft was removed from four of the trees. Small RNAs were sequenced from unexposed 'HoneySweet' trees, 'HoneySweet' trees that contained the graft as well as those that the infected graft had been removed. The goal was to determine the population of small RNAs related to Plum Pox Virus in 'HoneySweet' reative to the infected 'Emma Leppermann'. Samples were taken from leaf tissue above and below the graft as well as the graft, to determine if movement of the small RNAs took place.