U2OS Cell Cycle Transition Curve-- NCS 200 ng/mL

U2OS cells expressing the PCNA-mCherry reporter were induced with 200 ng/mL NCS at 20.4 hours into the movie and then imaged for another 48 hours. Time-lapse images were taken every 20 minutes.将表达PCNA-mCherry报告基因（PCNA-mCherry reporter）的U2OS细胞，于成像启动后20.4小时时以200 ng/mL的NCS进行诱导处理，随后继续成像48小时。成像采用延时拍摄模式，每20分钟采集一次图像。