Soluble varying proteins from tobacco leaf protein extracts identified by LC-MS/MS involved in CO₂ metabolism.

Soluble proteins were prepared from tobacco leaves of wild type plants, and plants over accumulating a Pseudomonas fluorescens p-hydroxyphenyl pyruvate dioxygenase (HPPD) or an Aequorea victoria GFP (GFP). Proteins have been analyzed by two-dimensional electrophoresis and identified by LC-MS/MS as described under Materials and Methods. Only spots containing a single protein have been considered. For more details about the listed proteins (sequences, accumulation levels, see Tables S1 and S2). Spot No., spot label; Accession number, accession number in NCBI database; Protein name, identified protein names; Organism, organism in which the protein has been identified; Subcellular localization, cellular compartment in which the protein has been identified; Function, protein function defined from literature; HPPD/WT and GFP/WT, patterns of variation when comparing spot volumes measured in HPPD or GFP proteome compared to wild type (WT) proteome, respectively: C, constant; D, down-accumulated; U, up-accumulated.