Drosophila enhancer adult bristle expression assay using UAS sv-RNAi

Data from manuscript \"Sex-specific evolution of a Drosophila sensory system via interacting cis- and trans-regulatory changes\" by D Luecke, G Rice, and A Kopp.

This study examines the regulation of expression for the chemosensory bristle specifier gene Pox neuro, which has expanded expression into ancestrally mechanosensory bristles in male D. prolongata. Flourescent assays show qualitativetly expanded expression driven by the D. prolongata allele relative to enhancers from sister species in the developing pupal leg. Adult bristle disruption assays using shavenoid RNAi show quantitative expression expansion by the D. prolongata allele into specific mechanosensory bristles on the dorso-anterior leg of the first tarsi.

本数据集源自D Luecke、G Rice与A Kopp撰写的学术论文《基于顺式与反式调控变异互作的果蝇感觉系统性别特异性演化》。

本研究针对化学感受刚毛特化基因Pox neuro的表达调控机制展开分析，该基因在长跗果蝇（D. prolongata）雄性个体中，表达范围已扩展至祖先源性的机械感受刚毛。

荧光测定实验结果显示，相较于近缘物种的增强子，长跗果蝇等位基因驱动的Pox neuro在发育蛹肢内的表达存在定性层面的扩展。

采用shavenoid RNA干扰（RNAi）开展的成虫刚毛功能干扰实验表明，长跗果蝇等位基因可将Pox neuro的表达定量扩展至第一跗节背前侧的特定机械感受刚毛。