Source data for Chen et al (2024) entitled "Motor Cortical Neuronal Hyperexcitability Associated with α-Synuclein Aggregation"

-------------------- GENERAL INFORMATION -------------------- This readme file was generated on [2024-01-15] by [Liqiang Chen]. Title of Dataset: Description of Dataset: Principal Investigator: Hong-Yuan Chu, [e-mail], ORCID: 0000-0003-0923-683X. Date of Data Collection: 2023-04-01 to 2024-11-10 Software Dependencies: Excel and Image J. ------------- FILE OVERVIEW ------------- Directory of Files: Source data, Electrophysiology trace data, and Microscopy images. Relationship Between Files: Source data is used to make figures in GraphPad. Electrophysiology trace data is used to plot electrophysiology traces. Microscopy images are used for representative images. File Formats: Microsoft Excel Worksheet (.xlsx) and confocal images (.nd2) File Naming Convention: Based on file formats. ---------------------------------------- DATA SPECIFIC INFORMATION FOR [Source data] Date of Creation: 2024-08-13 Description of Data: Source data is used to make figures in GraphPad. A. Missing data are represented n/a. B. Abbreviations (Primary cortex: M1; Secondary cortex: M2; α-Synulein: αSyn; intratelencephalic neurons: ITNs; corticospinal neurons: CSNs). C. Figure 5B data (ITN-Sholl analysis-Intersections-5 µm Radius) can not organized as tidy format because of too many data points in each group. DATA SPECIFIC INFORMATION FOR [Electrophysiology trace data] Date of Creation: 2024-08-13 Description of Data: Electrophysiology trace data is used to plot electrophysiology traces. A. Electrophysiology traces can be plotted using Excel. B. Traces are plotted in Electrophysiology trace data file. DATA SPECIFIC INFORMATION FOR [Microscopy images] Date of Creation: 2024-08-13 Description of Data: Microscopy images are used to make representative images. A. Microscopy images can be opened using Image J. B. Microscopy images are named based on experimental group, animal ID, and figure number in the manuscript. ----------- METHODOLOGY ----------- Description of methods used for data collection: Electrophysiology data is collected using MultiClamp 700B amplifier and Digidata 1550B. pClamp 11 software is used. Microscopy images are collected using an confocal microscope. Description of methods used for data processing: A. Electrophysiology data is processed using clampfit software, including measure the peak of EPSC, count the number of action potentials, measure the width/rise time/decay time of action potential. B. Microscopy images are processed using Image J software, including measure the α-Synulein pathologic area in motor cortex, quantify the TH staining, and verify the co-localization of pS129 and biocytin. C. All data after processed through clampfit and Image J is put into GraphPad to make figures. ----------------------- DATA ACCESS AND SHARING ----------------------- This research was funded in part by 1. Aligning Science Across Parkinson’s (ASAP-020572) through the Michael J. Fox Foundation for Parkinson’s Research (MJFF). 2. National Institute of Neurological Disorders and Stroke (R01NS121374). 3. Congressionally Directed Medical Research Programs (W81XWH-21-1-0943).# 通用信息 本说明文件由[陈立强]于[2024-01-15]生成。 数据集标题： 数据集描述： 项目负责人：朱宏远（Hong-Yuan Chu），邮箱：[e-mail]，开放研究者与贡献者身份识别码（ORCID）：0000-0003-0923-683X。 数据采集时间：2023-04-01 至 2024-11-10 软件依赖：Excel 与 Image J。 --- # 文件概览 文件目录：源数据、电生理轨迹数据、显微图像。 文件关联关系：源数据用于在GraphPad中绘制科研统计图；电生理轨迹数据用于绘制电生理轨迹图谱；显微图像用于制作代表性成像图片。 文件格式：Microsoft Excel 工作表（.xlsx）与共聚焦图像（.nd2） 文件命名规则：基于文件格式制定。 --- ## [源数据] 专项数据信息 创建日期：2024-08-13 数据说明：源数据用于在GraphPad中绘制科研统计图。 A. 缺失数据以“n/a”表示。 B. 术语缩写说明：初级皮层（M1）、次级皮层（M2）、α-突触核蛋白（αSyn）、端脑内投射神经元（ITNs）、皮质脊髓束神经元（CSNs）。 C. 图5B的数据（ITN-Sholl分析-交点-5μm半径）因每组数据点数量过多，无法整理为整洁数据格式（tidy format）。 --- ## [电生理轨迹数据] 专项数据信息 创建日期：2024-08-13 数据说明：电生理轨迹数据用于绘制电生理轨迹图谱。 A. 可通过Excel绘制电生理轨迹图。 B. 轨迹图已绘制于电生理轨迹数据文件中。 --- ## [显微图像] 专项数据信息 创建日期：2024-08-13 数据说明：显微图像用于制作代表性成像图片。 A. 可通过Image J打开显微图像。 B. 显微图像命名依据实验组、动物编号与稿件中的图号制定。 --- # 研究方法 ### 数据采集方法 电生理数据采用MultiClamp 700B放大器与Digidata 1550B采集设备进行采集，配套使用pClamp 11软件完成后续操作。显微图像通过共聚焦显微镜采集。 ### 数据处理方法 A. 电生理数据通过Clampfit软件处理，具体包括测量兴奋性突触后电流（EPSC）的峰值、计数动作电位数量、测定动作电位的时宽、上升时间与衰减时间。 B. 显微图像通过Image J软件处理，具体包括测量运动皮层内α-突触核蛋白的病理沉积区域、量化酪氨酸羟化酶（TH）的染色信号强度、验证pS129与生物胞素（biocytin）的共定位情况。 C. 经Clampfit与Image J处理后的全部数据将导入GraphPad以绘制最终统计图。 --- # 数据获取与共享 本研究部分经费来源于以下项目： 1. 迈克尔·J·福克斯帕金森症研究基金会（MJFF）资助的“帕金森症跨学科科学合作计划”（Aligning Science Across Parkinson’s, ASAP-020572）。 2. 美国国家神经疾病与卒中研究所（R01NS121374）。 3. 国会定向医学研究计划（W81XWH-21-1-0943）。