Transcriptome profile of MafB-cKO of podocyte

The transcription factor MafB is essential for differentiation and foot process formation of podocytes. In order to identify the downstream targets of MafB, we analyzed the Mafb-deficient podocyte by RNA-seq. We found slit diaphragm-related protein (Nphs1, Magi2), Rho GTPase-activating protein (Arhgap24, Iqgap2) and podocyte-specific transcription factor (Tcf21) were significantly reduced in MafB cKO glomeruli. This indicates that one of these factors might be directly regulated by MafB to maintain its function in podocyte maintenance. Examination of 6 samples (3 Control and 3 Mafb-cKO podocyte ). Each sample contains RNA from 1 mouse. Paired-end RNA sequencing was performed with NextSeq500 sequencer (Illumina).