Col2a1-Cre;Idh1LSL/+ embryonic growth plate chondrocytes - single cell RNA-sequencing

To determine differences between Idh1 mutant and control growth plates, we used scRNA sequencing. Three E18.5 growth plates from our Idh1 mutant mice and three control mice were studied. Differential gene expression analysis revealed genes that were expressed in the distinct clusters. In order to assess the Idh1 mutant expressing chondrocytes, we utilized Collagen2a1-Cre males crossed with Idh1 LSL/+ females. Growth plate chondrocytes from E18.5 distal part of femur and proximal part of tibia were collected and chondrocytes were harvested using Pronase I digestion followed by Collagenase IV. Live cells were sorted and GEM were made using 10xgenomics kit. After sequencing and downstream analysis using Seurat, we observed distinct clusters of cells with Idh1 mutation 10X Genomics Single cell RNA-sequencing of growth plate chondrocytes