Tregs modulate retention of T_RM cells within the brain following MCMV infection.

Mononuclear cells were harvested from Treg-sufficient and deficient mice, as previously described at 7, 14, & 30 dpi. CNS-derived lymphocytes were gated on CD8+ T-cells and analyzed for KLRG1 and CD103 (a marker for tissue resident-memory T-cells in the CNS). (A) Representative contour plots show expression levels of KLRG1 and CD103 gated on CD8+ T-cells at the indicated time points. (B) Pooled data show the MFI of CD8+ T-cells expressing CD103 at the indicated time points. Data are derived from 2 independent experiments using 2–4 animals per treatment group/time point. **p < 0.001 DTx- versus DTx+ MCMV-infected at all time points tested. (C) The number of CD103+CD8+ T-cells in infected brains with and without DTx treatment at 7, 14, &30 dpi is shown. **p < 0.001 DTx- versus DTx+