Exploring the vault interactome in the divergent eukaryote Trypanosoma brucei

Many, but not all Eukaryotes have protein-enclosed compartments called vaults. Vaults are composed of multiple copies of the major vault protein (MVP), symmetrically assembled into a basket like shell. Whilst there is some evidence that these compartments are engaging in mRNA maturation and intriguing structural information of the vault shell is available, the function of vaults remains largely elusive. Here, we set out to explore the vault interactome in the early branching eukaryote Trypanosoma brucei employing a combination of affinity capture and TurboID proximity labelling.