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Immunoprecipitation of cGAS/PCK1 and mass spectrometry analysis

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NIAID Data Ecosystem2026-05-02 收录
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BT-549 cells (5× 10^7) were treated with hypoxia treatment for 2 h. An immunoprecipitation assay was performed using anti-cGAS or anti-PCK1 antibody, and immunoprecipitates of cGAS or PCK1 were separated using SDS-PAGE and stained with Coomassie Brilliant Blue. Selected peptide hits of proteins associated with cGAS.

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2025-02-19
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