Strong pairwise interactions do not drive interactions in a plant leaf associated microbial community

Illumina amplicon sequencing of 16S and ITS2 regions of the SynCom on solid medium was performed for the investigation of the community composition in vitro (raw data: Metadata_amplicon_sequencing_for_metabolomics_syncom_only). In planta amplicon sequencing was used to analyse the effect of pseudobactin on the SynCom composition on plant leaves(raw data: Metadata_amplicon_sequencing_Pseudobactin_on_plant.xlsx). Correlation networks were created based on co-abundances of epiphytic microbiome members of the A. thaliana leaf. Data used for the analysis was collected by amplicon sequencing of 16S, ITS and 18S regions from A. thaliana plants sampled around Tübingen (Mahmoudi et al., 2023). A workflow on how the data was processed can be found in the readme file within the Microbial_Networks.zip file.本研究针对固体培养基上的合成微生物群落（Synthetic Community, SynCom）的16S与ITS2区域开展Illumina平台扩增子测序（amplicon sequencing），以体外探究其群落组成（原始数据：Metadata_amplicon_sequencing_for_metabolomics_syncom_only）。采用植物体内扩增子测序技术，分析假杆菌素（pseudobactin）对植物叶片上SynCom群落组成的影响（原始数据：Metadata_amplicon_sequencing_Pseudobactin_on_plant.xlsx）。 基于拟南芥（Arabidopsis thaliana, A. thaliana）叶片附生微生物组（epiphytic microbiome）成员的共丰度模式构建相关网络。本分析所用数据取自图宾根（Tübingen）周边采样的拟南芥植株，通过对其16S、ITS及18S区域进行扩增子测序获得（Mahmoudi等，2023）。数据处理流程可参见Microbial_Networks.zip压缩包内的README文件。