DNA sequencing at picogram level to investigate life on Mars and Earth

For studying life within the rocks of Earth and Mars, we demonstrate, in an ISO 5 clean room, a procedure based on MinION nanopore technology that correctly identifies organisms with only 2 pg of DNA without amplification, which represents unprecedented sensitivity. The folder contains fast5 and fastq passed and failed sequencing files of all the experiments conducted to investigate ONT's MinION ability to detect and characterize extremely low biomass.针对地球与火星岩石内部生命的研究，我们在ISO 5级洁净室（ISO 5 clean room）中验证了一套基于MinION纳米孔测序技术（MinION nanopore technology）的实验流程：该流程仅需2皮克DNA即可无需扩增完成生物体的准确鉴定，实现了前所未有的检测灵敏度。本数据集文件夹包含了所有相关实验的fast5与fastq格式测序通过文件及测序失败文件，这些实验旨在探究ONT的MinION技术检测并表征极低生物量的能力。