Eosinophil cationic protein is a potential surrogate marker of allergic contact dermatitis: A single-center, retrospective study of 216 patients

Supplement Table 1. Frequency of sensitization to allergens from the patch test in allergic contact dermatitis patients. Supplement Table 2. Correlations of total eosinophil count, total IgE, and disease duration with serum ECP in ACD and non-ACD groups. Supplement Table 3. Comparison of serum allergy markers between ACD and AD-ACD groups. Supplement Figure 1. (A) ROC curve testing whether serum ECP differentiates ACD from non-ACD patients (B) ROC curve testing of total IgE. ROC, Receiver operating characteristic; AUC, Area under curve Supplement Figure 2. Dermal eosinophil density from skin biopsy specimens of ACD patients was not correlated with their serum ECP levels (r = 0.21, p = 0.82). Among the study patients, 15 patients underwent a skin biopsy of lesional skin. Two board-certified dermatologists (J.H.K and Y.I.L) independently reviewed the hematoxylin and eosin sections. The presence of eosinophilic spongiosis and dermal eosinophil density were scored in a semi-quantitative manner. HPFs, High power fields.

补充表1. 变应性接触性皮炎（allergic contact dermatitis, ACD）患者斑贴试验过敏原致敏率。
补充表2. 变应性接触性皮炎与非变应性接触性皮炎组中，总嗜酸性粒细胞计数、总IgE及病程与血清嗜酸细胞阳离子蛋白（eosinophil cationic protein, ECP）的相关性。
补充表3. 变应性接触性皮炎与AD-ACD组血清过敏标志物的比较。
补充图1. (A) 检验血清ECP区分变应性接触性皮炎与非变应性接触性皮炎患者能力的受试者工作特征（Receiver operating characteristic, ROC）曲线；(B) 检验总IgE的受试者工作特征曲线。其中受试者工作特征（ROC），曲线下面积（Area under curve, AUC）。
补充图2. 变应性接触性皮炎患者皮肤活检标本的真皮嗜酸性粒细胞密度与其血清ECP水平无相关性（r=0.21，p=0.82）。本研究的受试患者中，共15例接受了皮损部位皮肤活检。两名经专科委员会认证的皮肤科医师（J.H.K与Y.I.L）独立阅片苏木精-伊红（hematoxylin and eosin, HE）染色切片。对嗜酸性海绵水肿表现与真皮嗜酸性粒细胞密度进行半定量评分。高倍视野（High power fields, HPFs）。