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Identification of Cross-linking partners of BpA modified p97 by photo cross-linking MS

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NIAID Data Ecosystem2026-05-01 收录
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We wanted to see where and how the protein p97 interacts with its partner proteins p37, PPI,Sds22 and I3. To this end a p97 derivative with genetically encoded crosslinking amino acid (p-benzoyl-L-phenylalanine) was generated. After incubation with the partner proteins, photocross-linking and tryptic digest the resulting cross-linked peptides were analysed by mass spectrometry.

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2023-07-10
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