Development of Delivery Systems with Prebiotic and Neuroprotective Potential of Industrial-Grade Cannabis sativa L.

Article, Dataset for article Abstract This study delves into the transformative effects of supercritical carbon dioxide (scCO2) cannabis extracts and prebiotic substances (dextran, inulin, trehalose) on gut bacteria, coupled with a focus on neuroprotection. Extracts derived from the Białobrzeska variety of Cannabis sativa, utilising supercritical fluid extraction (SFE), resulted in notable cannabinoid concentrations (cannabidiol (CBD): 6.675 ± 0.166; tetrahydrocannabinol (THC): 0.180 ± 0.006; cannabigerol (CBG): 0.434 ± 0.014; cannabichromene (CBC): 0.490 ± 0.017; cannabinol (CBN): 1.696 ± 0.047 mg/gD). The assessment encompassed antioxidant activity via four in vitro assays and neuroprotective effects against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). The extract boasting the highest cannabinoid content exhibited remarkable antioxidant potential and significant inhibitory activity against both enzymes. Further investigation into prebiotic deliveries revealed their proficiency in fostering the growth of beneficial gut bacteria while maintaining antioxidant and neuroprotective functionalities. This study sheds light on the active compounds present in the Białobrzeska variety, showcasing their therapeutic potential within prebiotic systems. Notably, the antioxidant, neuroprotective, and prebiotic properties observed underscore the promising therapeutic applications of these extracts. The results offer valuable insights for potential interventions in antioxidant, neuroprotective, and prebiotic domains. In addition, subsequent analyses of cannabinoid concentrations post-cultivation revealed nuanced changes, emphasising the need for further exploration into the dynamic interactions between cannabinoids and the gut microbiota. Keywords: Cannabis Sativa; SFE; antioxidant activity; neuroprotective activity; prebiotic; microbiome; industrial hemp研究论文：文章数据集 摘要 本研究探讨了超临界二氧化碳（supercritical carbon dioxide, scCO₂）大麻提取物与益生元物质（prebiotic substances，葡聚糖、菊粉、海藻糖）对肠道菌群的调控作用，并重点关注其神经保护活性。采用超临界流体萃取（supercritical fluid extraction, SFE）技术从比亚沃布热斯卡（Białobrzeska）品种的大麻（Cannabis sativa）中提取得到的提取物，其大麻素类物质含量可观：大麻二酚（cannabidiol, CBD）：6.675 ± 0.166；四氢大麻酚（tetrahydrocannabinol, THC）：0.180 ± 0.006；大麻萜酚（cannabigerol, CBG）：0.434 ± 0.014；大麻环萜酚（cannabichromene, CBC）：0.490 ± 0.017；大麻酚（cannabinol, CBN）：1.696 ± 0.047 mg/gD。本次评估通过四项体外实验检测了提取物的抗氧化活性，并考察了其对乙酰胆碱酯酶（acetylcholinesterase, AChE）与丁酰胆碱酯酶（butyrylcholinesterase, BChE）的神经保护作用。其中大麻素类物质含量最高的提取物，展现出优异的抗氧化潜能，且对上述两种酶均具有显著的抑制活性。针对益生元递送方式的进一步研究表明，此类物质可有效促进有益肠道菌群增殖，同时保留其抗氧化与神经保护功能。本研究揭示了比亚沃布热斯卡品种大麻中的活性成分，阐明了其在益生元体系中的治疗应用潜力。值得注意的是，本研究观测到的抗氧化、神经保护与益生元特性，进一步凸显了此类提取物在治疗领域的应用前景。本研究结果为抗氧化、神经保护及益生元相关的潜在干预手段提供了重要参考。此外，对种植后大麻素类物质浓度的后续分析显示出细微的变化，这强调了需进一步探究大麻素与肠道菌群之间的动态互作关系。 关键词：大麻（Cannabis sativa）；超临界流体萃取（supercritical fluid extraction, SFE）；抗氧化活性；神经保护活性；益生元（prebiotic）；微生物组（microbiome）；工业大麻（industrial hemp）