Data from: Three molecular markers show no evidence of population genetic structure in the Gouldian finch (Erythrura gouldiae)

Assessment of genetic diversity and connectivity between regions can inform conservation managers about risk of inbreeding, potential for adaptation and where population boundaries lie. The Gouldian finch (Erythrura gouldiae) is a threatened species in northern Australia, occupying the savannah woodlands of the biogeographically complex monsoon tropics. We present the most comprehensive population genetic analysis of diversity and structure the Gouldian finch using 16 microsatellite markers, mitochondrial control region and 3,389 SNPs from genotyping-by-sequencing. Mitochondrial diversity is compared across three related, co-distributed finches with different conservation threat-statuses. There was no evidence of genetic differentiation across the western part of the range in any of the molecular markers, and haplotype diversity but not richness was lower than a common co-distributed species. Individuals within the panmictic population in the west may be highly dispersive within this wide area, and we urge caution when interpreting anecdotal observations of changes to the distribution and/or flock sizes of Gouldian finch populations as evidence of overall changes to the population size of this species.

Usage Notes
Microsatellite DataMicrosatellite data.csvGBS Final Filtered VCFFinalFiltered.vcfmtDNA Arlequin Input FileStandard Arlequin including the haplotype numbers and their associated genbank accessions.
Also includes the pairwise geographic distances between sampling localities for use in the mantel test.mtDNA_Arlequin.arp

对遗传多样性和区域间连通性的评估，能够为保护管理者提供近亲繁殖风险、适应潜力以及种群边界所在之处的信息。金丝雀雀（Erythrura gouldiae）是一种在澳大利亚北部受到威胁的物种，栖息于生物地理上复杂的季风热带的草原林地中。我们利用16个微卫星标记、线粒体控制区域以及通过测序进行基因分型的3,389个单核苷酸多态性位点，对该物种的遗传多样性和结构进行了最全面的人口遗传学分析。我们将线粒体多样性在三种相关且共分布的金丝雀中进行了比较，这三种金丝雀具有不同的保护威胁状况。在分布范围的西部，任何分子标记都没有显示出遗传差异，而单倍型多样性低于一个常见的共分布物种，但丰富度并未降低。在西部的泛交种群中，个体可能在这个广阔区域内高度扩散，因此我们呼吁在解读关于金丝雀雀种群分布和/或群体规模变化的轶事观察结果时保持谨慎，这些结果不能作为该物种种群规模整体变化的证据。

使用说明

微卫星数据：Microsatellite Data.csv
GBS最终过滤的VCF文件：FinalFiltered.vcf
mtDNA Arlequin输入文件：Standard Arlequin，包括单倍型编号及其相关的genbank访问号。
还包括采样地点间的成对地理距离，用于曼特尔测试：mtDNA_Arlequin.arp