Small RNA profiles of follicular fluid-derived small extracellular vesicles

Follicular fluid (FF) provides a complex and suitable environment for oocyte maturation and contains several molecules secreted from oocyte and granulosa, cumulus, and theca cells. In addition, extracellular vesicles (EV) exist in various body fluids and are known as the cargo of several mRNAs, proteins, and miRNAs to communicate from cell to cell. In this study, we investigated the miRNA profiles of FF-derived EVs. Overall design: EVs were isolated from FF by the serial centrifugation method, and small RNA sequencing was performed.