Comparison of toxicological effects and exposure levels between triclosan and its structurally similar chemicals using in vitro tests for read-across case study. Comparison of toxicological effects and exposure levels between triclosan and its structurally similar chemicals using in vitro tests for read-across case study

To improve prediction accuracy of read-across, we compared biological similarities between triclosan and its structurally similar chemicals including diclosan and 1-chloro-3-(4-chlorophenoxy) benzene. To elucidate and compare the potential mechanisms between them, we performed in vitro toxicogenomics with rat primary hepatocytes. Overall design: We performed in vitro toxicogenomics with Sprague Dawley rat primary hepatocytes exposed by triclosan, diclosan, 1-chloro-3-(4-chlorophenoxy) benzene. After 24 h pre-culture, the cells were exposed to each chemical at 30 µM for 24 h. Three independent experiments were performed at each condition.