The nerve roots avulsion induced time-specific expression patterns of lncRNA and mRNA occurred before the motoneurons loss of the injured spinal segments

In this study, we study the expression pattern of the lncRNAs in rat’s spinal cord of at 3 days and 14 days after the unilateral BPRA. By comparing the expression patterns of mRNAs and lncRNAs, ipsilateral vs contralateral, on the 3rd and the 14th day, in the same spinal segments using microarray analysis. We identified 55 lncRNAs, which were significantly altered at the 3rd day after BPRA. Two weeks after BPRA, 70 lncRNAs which were significantly altered. Among these DE lncRNAs, 17 were consistently over-regulated at 3- and 14- days after injury while 5 lncRNAs were consistently down-regulated. At 3-days after injury, 399 mRNAs were significantly differentially expressed while two weeks after BPRA, 445 were downregulated. Among these mRNAs, 198 were consistently over-regulated, and 15 mRNAs were consistently under-regulated. The brachial plexus root avulsion model (C5-T1) were builted, then we study the expression pattern of the lncRNAs in rat’s spinal cord of at 3 days and 14 days after the brachial plexus root avulsion.